Plasmid DNA Manufacture Service
We provide high-quality plasmid DNA Manufacture Services. Customers can select scales of production to best match their needs.
Features
- Low Endotoxin level
- High proportion of supercoiled DNA
- Strict quality control system and lot release specification
- Product delivered in lyophilized powder to maximize stability and solubility
Service scales and applications
We offer customized plasmid manufacture services at scales from milligrams to grams using GeneImmune’s unique and proprietary process that has been fully validated through the cGMP manufacture of clinical grade plasmid products. Plasmid DNA purified by GeneImmune is suitable for:
- Preclinical studies of DNA vaccine or gene therapy including toxicology and biodistribution studies
- DNA vaccine research
- Gene therapy research
- Transfection experiments
- Other research applications
Quality control and lot release
GeneImmune produces and delivers plasmid DNA of DNA-Gold and DNA-Platinum grades that meet your highest quality demands for the purpose of transfection, animal immunization and preclinical studies for DNA vaccine or gene therapy products.
For an small extra fee, additional quality control tests are also available from our QC team to ensure qualification for most demanding applications:
Test | Lot Release Specification for DNA-Gold Grade | Lot Release Specification for DNA-Platinum Grade | Price |
Appearance | Clear, no foreign particles | Clear, no foreign particles | Free |
A260/A280 | 1.75 - 2.0 | 1.75 - 2.0 | Free |
Percentage of Supercoiled DNA (Agarose gel) | Predominantly Supercoiled | ≥ 80% Supercoiled guaranteed | Free |
Identity(Restriction analysis) | Co-migrates with standard or as specified by client | Co-migrates with standard or as specified by client | Free |
RNA residual | Not detectable on agarose gel | Not detectable on agarose gel | Free |
E.coli Genomic DNA (Slot Blot or Real-time Q-PCR) | ≤ 20μg/mg plasmid | ≤ 2μg/mg plasmid | Free |
Host Cellular Protein (ELISA or BCA) | ≤ 20μg/mg plasmid | ≤ 1μg/mg plasmid | Free |
Endotoxin (LAL) | ≤ 100EU/mg | ≤ 100EU/mg | Free |
Test | Method | Price |
Bioburden Assay | LB agar plate | Please inquire |
Gene Expression | Western blot (with specified antibody from IT or client) | Please inquire |
Humoral Immunity | ELISA (with specified antigen from IT or client) | Please inquire |
Cellular Immunity | ELISPOT or ICS (with specified peptides from IT or client) | Please inquire |
Your Test | Your choice | Please inquire |
Product Catalog
Product scale | DNA-Gold Grade | DNA-Platinum Grade | ||
Catalog # | Price | Catalog # | Price | |
5 milligrams | GI-D005G | Please inquire | GI-D005P | Please inquire |
10 milligrams | GI-D010G | Please inquire | GI-D010P | Please inquire |
20 milligrams | GI-D020G | Please inquire | GI-D020P | Please inquire |
100 milligrams | GI-D100G | Please inquire | GI-D100P | Please inquire |
> 100 milligrams | GI-D999G | Please inquire | GI-D999P | Please inquire |
250 mL(Midiprep) | GI-000G | Please inquire | ||
1 liter(Maxiprep) | GI-L001G | Please inquire | ||
5 liter | GI-L005G | Please inquire | ||
10 liter | GI-L010G | Please inquire |
Yield for volume-based orders are not guaranteed and depend on the characteristic of individual plasmid. Typical yield for high-yield plasmids is >3 mg/liter of LB).
Contact us
If you like to purchase, or need any assistance, please do not hesitate to contact us at:
GeneImmune Biotechnology Corp.
15601 Crabbs Branch Way
E128
Rockville, MD 20855
Tel. 301-861-4366
Order: order@geneimmuneusa.com
Info: info@geneimmuneusa.com
15601 Crabbs Branch Way
E128
Rockville, MD 20855
Tel. 301-861-4366
Order: order@geneimmuneusa.com
Info: info@geneimmuneusa.com