Protein Production Service
Time frames
A. HEK-293 Mammalian System
Feature: Best choice for expressing almost any protein, with best success rate for secreted proteins
Phases | Time |
Expression vector construction | 1 week |
Amplification of recombinant Plasmid | 1 week |
Protein production | 1 week |
Purification | 1-2 weeks |
Total | 4-5 weeks |
B. Bacterial System
Feature: Cost effective; protein size up to 250 kDa
Phases | Time |
Expression vector construction | 1-2 weeks |
Generation and selection of bacterial colonies expressing target protein | 1-2 weeks |
Culture of recombinant bacteria | 2 days |
Purification | 2-3 weeks |
Total | 4-7 weeks |
C. Baculoviral System
Feature: Protein expressed in this system lacks most post-translational modifications; nearly unlimited protein size
Phases | Time |
Expression vector construction | 1-2 weeks |
Generation and selection and plaque purification of baculoviral colonies expressing target protein | 2-3 weeks |
Amplification of recombinant baculovirus; generation of high-titer virus stock | 1 week |
Protein production | 1 week |
Purification | 1-2 weeks |
Total | 5-9 weeks |
D. Yeast System
Feature: Cost effective
Phases | Time |
Expression vector construction | 1-2 weeks |
Generation and selection of yeast colonies expressing target protein | 1-2 weeks |
Protein production | 1 week |
Purification | 2-3 weeks |
Total | 5-8 weeks |
Product Catalog
Service description | Catalog # | Price |
Set-up fee (includes the cost for constructing the expression vector and testing the protein expression) | GI-P100 | Please inquire |
Mammalian/Baculoviral System: 1mg | GI-P110-1MG | Please inquire |
Mammalian/Baculoviral System: 1-5mg | GI-P110-5MG | Please inquire |
Mammalian/Baculoviral System: 5-10mg | GI-P110-10MG | Please inquire |
Mammalian/Baculoviral System: 10-20mg | GI-P110-20MG | Please inquire |
Mammalian/Baculoviral System: >20mg | GI-P110-C | Please inquire |
Bacterial/Yeast System: 1mg | GI-P120-1MG | Please inquire |
Bacterial/Yeast System: 1mg | GI-P120-1MG | Please inquire |
Bacterial/Yeast System: 1-5mg | GI-P120-5MG | Please inquire |
Bacterial/Yeast System: 5-10mg | GI-P120-10MG | Please inquire |
Bacterial/Yeast System: 10-20mg | GI-P120-20MG | Please inquire |
Bacterial/Yeast System: >20mg | GI-P120-C | Please inquire |
Guaranteed >95% purity for secreted protein and >75% purity for intracellular proteins
Contact us
If you like to purchase, or need any assistance, please do not hesitate to contact us at:
15601 Crabbs Branch Way
E128
Rockville, MD 20855
Tel. 301-861-4366
Order: order@geneimmuneusa.com
Info: info@geneimmuneusa.com